|
| Status |
Public on Apr 02, 2015 |
| Title |
Kidney_donor_DCD_T1_35 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney_donor_DCD_T1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: kidney biopsy
donor type (0=living, 1=bd, 2=nhb): 2
timepoint biopsy: 1
deceased donors, only left kidney included in the analysis: 1
delayed graft function (0=no, 1=yes): 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality was determined by high throughput Caliper GX LabChip RNA kit (Caliper).
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5937329004_A
|
| Data processing |
The raw data files were processed using GenomeStudio Software and further analyzed using Genespring GX 12.0 software (Agilent, Santa Clara, USA). Data normalization was performed using the default GeneSpring GX 12.0 median shift normalization to the 75 percentile (applying a log2 transformation) and baseline transformation using the median of all samples.
|
| |
|
| Submission date |
Jan 31, 2013 |
| Last update date |
Apr 02, 2015 |
| Contact name |
Jeffrey Damman |
| Organization name |
University Medical Center Groningen
|
| Department |
Pathology
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE43974 |
Pathways for intervention to optimize donor organ quality uncovered: a genome wide gene expression study |
|
