|
| Status |
Public on Jan 01, 2014 |
| Title |
Classical 1A_5 |
| Sample type |
RNA |
| |
|
| Source name |
whole blood, classical type 1A
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
gender: female
age: 76yrs
diabetes type: classical type 1A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood samples were collected in PAXgene Blood RNA tubes (PreAnalytiX, Hombrechtikon, Switzerland), incubated and stored according to the manufacturer’s protocol. Total RNA was extracted using the PAXgene Blood RNA Kit (PreAnalytiX) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA was synthesized from 250 ng of total RNA using the Quick Amp Labeling Kit, One-Color (Agilent Technologies).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to an Agilent Whole Human Genome Oligo Microarray 44k (Design ID: 014850).
|
| Scan protocol |
All procedures from labeling to scanning were performed according to the manufacturer’s instructions.
|
| Description |
Gene expression in human blood
|
| Data processing |
Intensity values for each scanned feature were quantified using Agilent Feature Extraction Software, which performs background subtractions. Normalization was performed using Agilent GeneSpring GX version 11.0.2. (per chip normalization: 75 percentile shift; per gene normalization: median of all samples).
|
| |
|
| Submission date |
Feb 13, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Shinsuke Nakata |
| E-mail(s) |
nakata@endmet.med.osaka-u.ac.jp
|
| Phone |
81-6-6879-3732
|
| Fax |
81-6-6879-3739
|
| Organization name |
Osaka University, Graduate School of Medicine
|
| Department |
Metabolic Medicine
|
| Street address |
2-2-B5, Yamadaoka
|
| City |
Suita |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE44313 |
Gene expression Fulminant type 1 diabetes vs classical type 1A diabetes |
|
| Relations |
| Reanalyzed by |
GSM1082973 |
