|
Status |
Public on Jul 03, 2013 |
Title |
bERKO Granulosa PMSG biological rep2 |
Sample type |
RNA |
|
|
Source name |
PMSG treated granulosa cells from bERKO mice
|
Organism |
Mus musculus |
Characteristics |
cell type: preovulatory granulosa cells treatment: 48h after PMSG genotype: bERKO
|
Treatment protocol |
Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
|
Growth protocol |
Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
|
|
|
Hybridization protocol |
5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
|
Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
Description |
Gene expression data from bERKO granulosa cells treated for 48h with PMSG
|
Data processing |
Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
|
|
|
Submission date |
Feb 25, 2013 |
Last update date |
Jul 03, 2013 |
Contact name |
NIEHS Microarray Core |
E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
Organization name |
NIEHS
|
Department |
DIR
|
Lab |
Microarray Core
|
Street address |
111 T.W. Alexander Drive
|
City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE44651 |
Laser Capture Microdissection isolation of preovulatory granulosa cells from WT and bERKO ovaries |
|