|
| Status |
Public on Jul 03, 2013 |
| Title |
WT Granulosa hCG biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
PMSG/hCG treated granulosa cells from WT mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: preovulatory granulosa cells
treatment: 48h after PMSG + 4 h after hCG
genotype: WT
|
| Treatment protocol |
Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
|
| Growth protocol |
Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
|
| |
|
| Hybridization protocol |
5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
Gene expression data from WT granulosa cells treated for 48h with PMSG followed by 4h with hCG
|
| Data processing |
Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
|
| |
|
| Submission date |
Feb 25, 2013 |
| Last update date |
Jul 03, 2013 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE44651 |
Laser Capture Microdissection isolation of preovulatory granulosa cells from WT and bERKO ovaries |
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