|
| Status |
Public on Apr 08, 2013 |
| Title |
Wild-type_Kidney_medulla_1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type_Kidney_medulla
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
gender: male
genotype/variation: Wild-type
age: 8-11 wks
tissue: Kidney medulla
|
| Treatment protocol |
SAGE knockout of Mdr1a, Mrp2, Bcrp (www.sageresearchmodels.com)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen RNeasy columns according to the manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
|
| Description |
Male wild-type Sprague-Dawley rats 8-11 weeks old
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 1500.
|
| |
|
| Submission date |
Mar 07, 2013 |
| Last update date |
Apr 08, 2013 |
| Contact name |
Keith M Goldstein |
| E-mail(s) |
goldsteinkm@lilly.com
|
| Organization name |
Eli Lilly & Co.
|
| Department |
Investigative Toxicology
|
| Lab |
Keith Goldstein
|
| Street address |
Lilly Corporate Center
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46285 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE44962 |
Minor compensatory changes in SAGE Mdr1a (P-gp), Bcrp, and Mrp2 knockout rats do not detract from their utility in the study of transporter-mediated pharmacokinetics. |
|
