|
| Status |
Public on Dec 31, 2014 |
| Title |
SCC10 |
| Sample type |
RNA |
| |
|
| Source name |
skin squamous cell carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: skin squamous cell carcinoma
|
| Treatment protocol |
Human SCC samples were obtained with Massachusetts General Hospital institutional approval as discarded material during Mohs micrographic surgery. For controls, normal skin was shaved very superficially with a #15 scalpel blade with minimal dermal contamination.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
15 μg total RNA from normal epidermis and SCC lesions (all from different patients) were used for RNA probe preparation and hybridization to gene chips (Human Genome U133A 2.0 array; Affymetrix) according to the manufacturer’s recommendations
|
| Label |
biotin
|
| Label protocol |
The resulting cDNAs were used for preparation of biotin-labeled antisense cRNA
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was hybridized on Human Genome U133A 2.0 array according to the manufacturer’s recommendation
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
Raw data were analyzed using the RMA package, available within Bioconductor.
|
| |
|
| Submission date |
Mar 14, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
giovanna chiorino |
| E-mail(s) |
giovanna.chiorino@gmail.com
|
| Organization name |
Fondo Edo Tempia
|
| Department |
Cancer Genomics
|
| Street address |
via malta 3
|
| City |
Biella |
| ZIP/Postal code |
13900 |
| Country |
Italy |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE45164 |
Transcription profiling of human skin squamous cell carcinoma (SCC) |
|
