|
| Status |
Public on Aug 17, 2014 |
| Title |
week13-pbspump-2 |
| Sample type |
RNA |
| |
|
| Source name |
skin- week 12/13 tx
|
| Organism |
Mus musculus |
| Characteristics |
treatment: PBS- pump
weeks of treatment: 12/13
strain: C3H/HeJ
tissue: skin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNeasy with on-column DNase digestion
|
| Label |
biotin
|
| Label protocol |
3' IVT Express Kit, Affymetrix
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G3000.
|
| Data processing |
The data were analyzed with Bioconductor/R and were RMA normalized.
|
| |
|
| Submission date |
Mar 27, 2013 |
| Last update date |
Aug 17, 2014 |
| Contact name |
Ali Jabbari |
| Organization name |
University of Iowa
|
| Street address |
200 Hawkins Dr, 40025 PFP
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE45551 |
Prevention of Mouse AA with IL-15 pathway inhibitors |
|
