|
| Status |
Public on Dec 08, 2014 |
| Title |
Healthy control PBMCs, non-treated, 5 |
| Sample type |
RNA |
| |
|
| Source name |
Control PBMCs from healthy human, not incubated with Pioglitazone
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: healthy
cell type: PBMCs
treatment: none
|
| Treatment protocol |
Cells were incubated in the presence or absence of Pioglitazone for 6 hours prior to RNA harvesting.
|
| Growth protocol |
Freshly isolated PBMCs were cultured in RPMI/10% FBS in the presence or absence of 1µM pioglitazone (Sigma-Aldrich, St. Louis, MO, USA) for various timepoints. For microarray studies, PBMCs were cultured with pioglitazone for 6 h, harvested and RNA isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with TriPure (Roche). For microarray analysis, RNA was further purified and concentrated using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Biotin-labeled cDNA was fragmented and hybridized to Affymetrix Human U133 Plus 2.0 GeneChip arrays (Santa Clara, CA) according to the manufacturer's protocol.
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Ctrl_5
Gene expression data from healthy control PBMCs not incubated with Pioglitazone.
|
| Data processing |
The data were analyzed using the GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 10 were used for the normalization.
|
| |
|
| Submission date |
Apr 09, 2013 |
| Last update date |
Dec 09, 2014 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL11670 |
| Series (1) |
| GSE45923 |
The peroxisome proliferator-activated receptor-gamma agonist pioglitazone modulates aberrant T cell responses in systemic lupus erythematosus |
|
