|
| Status |
Public on Jul 01, 2013 |
| Title |
0 Gy, 0 hr (3) |
| Sample type |
genomic |
| |
|
| Source name |
MDA-MB-231 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
gender: female
ionizing radiation treatment: 0 Gy, 0 hr (3)
disease state: breast cancer
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen EZ1 Advanced
|
| Label |
Cy3 and Cy5
|
| Label protocol |
The standard Infinium HD Methylation Assay protocol was used.
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented, and hybridized to the Illumina Infinium HumanMethylation450 Beadchip using the standard Infinium HD Methylation Assay protocol.
|
| Scan protocol |
Arrays were scanned on an Illumina iScan using Illumina recommended settings.
|
| Description |
SZ-3
|
| Data processing |
GenomeStudio v2011.1. Average Beta (Normalized using 'Controls' method; background subtracted). Unmethylated and methylated signal intensities (Normalized using 'Controls' method; background subtracted).
|
| |
|
| Submission date |
Apr 10, 2013 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Steven P Zielske |
| Organization name |
Wayne State University
|
| Department |
Department of Radiation Oncology
|
| Street address |
540 E. Canfield
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE45958 |
Radiation-induced epigenetic DNA methylation modification of radiation-response pathways |
|
