|
| Status |
Public on Jan 01, 2014 |
| Title |
SKNAS Vehicle |
| Sample type |
RNA |
| |
|
| Source name |
SKNAS cells treated with vehicle for 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKNAS
myc status: c-Myc
treatment: vehicle for 48h
|
| Treatment protocol |
Cells were either treated with vehicle or 2.5 micromolar ciclopirox for 48 hours.
|
| Growth protocol |
Cells were cultured in RPMI medium supplemented with 10% FCS, 1% L-glutamine, 1% penicillin-streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 3' IVT Express protocol starting with 100 ng of total RNA (GeneChip 3' IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan system.
|
| Description |
jyo009
|
| Data processing |
Data were background adjusted, normalized and summarized by the RMA method.
|
| |
|
| Submission date |
Apr 10, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
JUN YANG |
| E-mail(s) |
jun.yang2@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Surgery
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (2) |
| GSE45969 |
Targeting Myc signaling pathway by inhibition of histone demethylase JMJD2B (ciclopirox) |
| GSE45970 |
Targeting Myc signaling pathway by inhibition of histone demethylase JMJD2B |
|
