|
| Status |
Public on Feb 24, 2014 |
| Title |
Matched Control Group [cv000593 3months] |
| Sample type |
RNA |
| |
|
| Source name |
Blood - PaxGene
|
| Organism |
Homo sapiens |
| Characteristics |
group: Matched Control Group
age: 59.96
gender: Male
cad: No
diabetes: No
1 year weight loss (%): 0.59
|
| Treatment protocol |
Participants were enrolled on an ongoing basis in a lifestyle intervention that consisted of four components: 1) a very low fat vegetarian diet (<10% of calories from fat); 2) 180 minutes/week of moderate aerobic exercise; 3) one hour of stress management each day; and 4) weekly group support sessions (40). Clinical staff met with patients twice each week during the first 12 weeks to orient participants to the program and maximize adherence. The remainder of the program was primarily self-directed but included ongoing weekly stress management and group support sessions.
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen PAXgene™ Blood RNA System
|
| Label |
Biotin
|
| Label protocol |
double-stranded cDNA (1 ug) labeled with biotin-11-UTP using MessageAmp™ II aRNA Amplification System (Ambion)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C onGeneChip® Human Genome U133A 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
scanned on a GeneChip® Scanner 3000.
|
| Description |
Gene Expression Data from a matched Control Group participant
|
| Data processing |
Partek® Genomics Suite v6.5 (Partek Incorporated) was used to analyze gene expression data from the 378 CEL files, which all passed standard QC assessment. Duplicate blood samples collected at each time point from 7 random participants indicated high repeatability of the microarray data (average Pearson correlation of normalized intensities was 0.992 + 0.006, range 0.969-0.996). Paired t-tests identified 9 genes that were excluded from further analysis because they showed significant differences in expression among duplicate samples based on a false discovery rate (FDR) adjusted P-value <0.05
|
| |
|
| Submission date |
Apr 16, 2013 |
| Last update date |
Feb 20, 2015 |
| Contact name |
Darrell Ellsworth |
| E-mail(s) |
d.ellsworth@wriwindber.org
|
| Organization name |
Windber Research Institute
|
| Street address |
620 Seventh St.
|
| City |
Windber |
| State/province |
PA |
| ZIP/Postal code |
15963 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE46097 |
Expression data of Participants of Ornish intervention and Control group |
| GSE66175 |
Imporatance of substantial weight loss for altering gene expression during intensive cardiovascular lifestyle modification |
|
