|
| Status |
Public on May 11, 2013 |
| Title |
HUVEC MEF2 A/C SI.2 |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUVEC
genotype/variation: MEF2A/C
|
| Treatment protocol |
Transfected with siRNAs (Stealth siRNA, Invitrogen) for 72 hours
|
| Growth protocol |
HUVECs grown in EGM-2 (Lonza)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNeasy per manufacturer's protocol
|
| Label |
CY3
|
| Label protocol |
Samples were labeled according to gene expression profiling protocol from Illumina
|
| |
|
| Hybridization protocol |
Samples were hybridized onto beadchips according to gene expression profiling protocol from Illumina.
|
| Scan protocol |
The Illumina BeadArray Reader and BeadScan software were used to scan and extract raw intensity values.
|
| Data processing |
Background subtraction and quantile normalization were performed.
|
| |
|
| Submission date |
May 10, 2013 |
| Last update date |
May 11, 2013 |
| Contact name |
hyung chun |
| E-mail(s) |
hyung.chun@yale.edu
|
| Organization name |
Yale University
|
| Department |
Internal Medicine
|
| Street address |
300 George St Room 770H
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE46825 |
Apelin/APJ signaling mediated by G alpha 13 and MEF2 in endothelial cells |
|
