|
| Status |
Public on Jun 20, 2013 |
| Title |
Sendai Virus Infected with miR-203 Mimic Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
infection: Sendai virus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using Qiagen's miRNEasy Kit
|
| Label |
Cy3
|
| Label protocol |
standard as recommended by illumina
|
| |
|
| Hybridization protocol |
standard as recommended by illumina
|
| Scan protocol |
standard as recommended by illumina
|
| Description |
A549 cells were transfected with 50nM miR-203 mimic using Lipofectamine 2000. Fifteen hours after transfection, cells were infected with Sendai virus (Cantell strain, 5 pfu/cell) for 10 hours prior to RNA purification.
|
| Data processing |
quantile normalization
|
| |
|
| Submission date |
Jun 03, 2013 |
| Last update date |
Jun 20, 2013 |
| Contact name |
William Buggele |
| Organization name |
Northwestern University
|
| Street address |
2200 Campus Dr.
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE47865 |
Identification of miR-203 Regulated Genes During Sendai Virus Infection |
|
