|
| Status |
Public on Dec 16, 2013 |
| Title |
Spatial Aged-Unimpaired 5 |
| Sample type |
RNA |
| |
|
| Source name |
Spatial Aged-Unimpaired
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: CA3 Hippocampus
|
| Treatment protocol |
Spatial or Non-spatial task
|
| Growth protocol |
Aged rats, microdissected CA3 region of hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by homogenization in TRIzol reagent (Invitrogen) followed by application to Qiagen RNeasy columns.
|
| Label |
biotin
|
| Label protocol |
as per manufacture's recommendation
|
| |
|
| Hybridization protocol |
as per manufacture's recommendation
|
| Scan protocol |
as per manufacture's recommendation
|
| Data processing |
Using the the R/Bioconductor system data were normalized and summarized using the affy and gcRMA packages. One array was dropped as an outlier. A known batch effect was removed by subtracting the average batch deviation from the mean on an individual probe basis. An empirical low expression cutoff of 5 units was used to remove noise from the data used here, leaving 16204 of the original 31099 probe sets in the analysis.
|
| |
|
| Submission date |
Jun 12, 2013 |
| Last update date |
Dec 17, 2013 |
| Contact name |
Carlo Colantuoni |
| E-mail(s) |
ccolantu@jhmi.edu
|
| Phone |
4104931439
|
| Organization name |
Johns Hopkins Univ. School of Medicine
|
| Department |
Neurology
|
| Street address |
733 N Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE47867 |
Behaviorally activated mRNA expression profiles produce signatures of learning and enhanced inhibition in aged rats with preserved memory |
|
