|
| Status |
Public on Jun 18, 2013 |
| Title |
WholeBloodRNA_IN0148_Day1 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: whole blood
subject: IN0148
time: Day1
treatment: trivalent influenza vaccination
|
| Growth protocol |
For each sample, 2.5 mL whole blood was collected directly into PAXgene RNA Stabilization Tubes (QIAGEN), mixed by inverting, incubated at room temperature for at least 2 hours, then frozen at -80C until the time of purification
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN PAXgene Blood RNA System, in accordance with the protocol provided with the kit. Quality control was performed on a BioRad Experion microfluidic electrophoresis system
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol. Scanning was performed on an Illumina iScan system
|
| Data processing |
Raw data underwent background adjustment, variance stabilization transformation, and robust spline normalization using R package lumi (available from Bioconductor) and function lumiExpresso()
The data normalized by background adjustment, variance stabilization transformation, and robust spline normalization in R package lumi
|
| |
|
| Submission date |
Jun 17, 2013 |
| Last update date |
Dec 03, 2013 |
| Contact name |
Luis M Franco |
| Organization name |
National Institutes of Health
|
| Department |
National Institute of Allergy and Infectious Diseases
|
| Lab |
Laboratory of Immune System Biology
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE48018 |
Time series of global gene expression after trivalent influenza vaccination in humans (male cohort) |
| GSE48024 |
Time series of global gene expression after trivalent influenza vaccination in humans |
|
