|
| Status |
Public on Dec 15, 2015 |
| Title |
RPTEC clone 3 +INF-alpha |
| Sample type |
RNA |
| |
|
| Source name |
RPTECs_INF-alpha stimulated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: kidney
cell type: human renal proximal tubular epithelial cells (RPTECs)
stimulated with: 100U/ml INF-alpha for 48 hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNA easy Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions and quantified by NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Inc.) and its quality was assessed by Agilent 2100 Bioanalyzer (Agilent Technologies), and only samples with RNA integrity number >8.5 were used.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5083896035_J
|
| Data processing |
The data were normalised using Shift to 75 percentile with Genespring 11.0 (baseline transformation: median of all samples)
Please note that the data in the sample data table just normalized values without additional processing/normalization. However, additional processing/normalization procedure were performed to filter statistical significant probes.
|
| |
|
| Submission date |
Jul 05, 2013 |
| Last update date |
Dec 15, 2015 |
| Contact name |
Fabio Sallustio |
| E-mail(s) |
f.sallustio@nephro.uniba.it
|
| Organization name |
University of Bari
|
| Street address |
Piazza G. Cesare N°11
|
| City |
Bari |
| State/province |
Bari |
| ZIP/Postal code |
70124 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE48551 |
Role of interferon-alpha in the activation of tubular epithelial cell in lupus nephritis (LN) |
|
