|
| Status |
Public on Aug 01, 2006 |
| Title |
SPC-HA x TCR-HA_2 |
| Sample type |
RNA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
genetic modification: Isolated from double transgenic mice expressing influenza hemagglutinin (HA) under the transcriptional control of the surfactant protein C promotor and an MHC class II restricted alpha beta TCR specific for the HA peptide 110-120.
tissue: alveolar epithelial cell type II
|
| Treatment protocol |
Dispase/Dnase digestion of the lung tissue to release AEC II followed by untouched fluorescence activaed cell sorting
|
| Growth protocol |
ex vivo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000.
|
| Description |
SPC-HA x TCR-HA_2
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jul 13, 2006 |
| Last update date |
Jul 13, 2006 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE5300 |
Expression data from alveolar type II epithelial cells |
|
