tissue: liver parenchyma paired non-tumor nodules from a patient with HBV-positive hapatocellular carcinoma ,whose ID number is HQW. HBV, hepatitis B
Extracted molecule
total RNA
Extraction protocol
Total RNAs are harvested using TRIzol (Invitrogen) and the RNeasy kit (Qiagen) according to manufacturer’s instructions, including a DNase digestion step.
Label
cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
Scan protocol
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Description
This array contains ~19000 RefSeq coding genes and ~21000 long non-coding RNAs (lncRNA).
Data processing
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
Submission date
Aug 09, 2013
Last update date
Aug 10, 2013
Contact name
Wang Kang
Organization name
Eastern Hepatobiliary Surgery Hospital Second Military Medical University
