|
| Status |
Public on Aug 30, 2013 |
| Title |
zip-2 OP50 - rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
zip-2 uninfected
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
age: young adult
time: 4 hours
|
| Treatment protocol |
Animals were transferred to SK plates seeded with E. coli OP50 or P. aeruginosa PA14
|
| Growth protocol |
C. elegans N2 were added as L1 larvae to RNAi plates seeded with L4440 control bacterial or zip-2 RNAi bacteria at 20C for 48 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TriReagent followed by Rneasy column clean-up
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol at Harvard Medical School Biopolymers Facility per their protocol in 2008
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr on GeneChip Celegans Genome Array at Harvard Medical School Biopolymers Facility per their protocol in 2008
|
| Scan protocol |
GeneChips were scanned at Harvard Medical School Biopolymers Facility per their protocol in 2008
|
| Description |
Gene expression data from zip-2 RNAi uninfected animals grown on E. coli
|
| Data processing |
Data were analyzed at Harvard Medical School Biopolymers Facility per their protocol in 2008
|
| |
|
| Submission date |
Aug 30, 2013 |
| Last update date |
Aug 30, 2013 |
| Contact name |
Emily Troemel |
| E-mail(s) |
etroemel@ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Department |
Cell and Developmental Biology
|
| Lab |
Troemel
|
| Street address |
9500 Gilman Dr, #0349
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE50513 |
Identify genes regulated by zip-2 in absence and presence of P. aeruginosa PA14 infection at 4h |
|
