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Status |
Public on Sep 20, 2016 |
Title |
Palapattu_014_UDP_MDV2 |
Sample type |
RNA |
|
|
Source name |
C4-2 cells after 96 hours of UDP-GlcNAc + MDV3100 treatment
|
Organism |
Homo sapiens |
Characteristics |
cell line: C4-2 cell type: Prostate cancer
|
Treatment protocol |
100,000 cells/well in 24 well plate were grown and 15 mM UDP-N-acetylglucosamine or 10uM MDV 3100 alone or in combination were added to the cells for 96 hours
|
Growth protocol |
C42 cells were grown in grown in T-media with 10 % Fetal Bovine with and 1% PenStrip
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Extracted RNA samples were send for microarray using Human Affymetrix Gene ST 2.1
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Affymetrix Gene ST 2.1 . Chips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Data processing |
Gene expression analysis with values for each gene being calculated using robust multi-array average (RMA).
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Submission date |
Sep 06, 2013 |
Last update date |
Sep 20, 2016 |
Contact name |
Akash Kumar Kaushik |
E-mail(s) |
akkaushi@bcm.edu
|
Phone |
9362050781
|
Organization name |
Baylor College of Medicine
|
Department |
Molecular and Cellular Biology
|
Lab |
Sreekumar Lab 120D
|
Street address |
One Baylor Plaza
|
City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL17692 |
Series (1) |
GSE50661 |
Pathway based integrative analysis reveals a key role for the hexosamine biosynthetic pathway in prostate cancer progression with therapeutic implications |
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