|
| Status |
Public on Sep 20, 2013 |
| Title |
6969568028_R06C02 |
| Sample type |
genomic |
| |
|
| Source name |
Buffy coat
|
| Organism |
Homo sapiens |
| Characteristics |
gender: F
age: 58.845
age at menarche: 14
cancer type (icd-10): C50
time to diagnosis: 3.896
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from peripheral blood leukocytes using the QIAsymphony DNA Mini Kit (QIAGEN) according to the manufacturer's instructions
|
| Label |
Biotin and 2,4-DNP
|
| Label protocol |
Standard Illumina Infinium HD Methylation assay protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA (using the EZ-96 DNA Methylation-Gold Kit, Zymo Research) was amplified, fragmented and hybridized to the Illumina Infinium HumanMethylation450 BeadChip using the standard manufacturer's protocol
|
| Scan protocol |
Arrays were imaged using the Illumina HiScanSQ system using standard recommended scanner setting
|
| Data processing |
Background subtraction and dye bias correction were performed using in-house routines
|
| |
|
| Submission date |
Sep 20, 2013 |
| Last update date |
Sep 20, 2013 |
| Contact name |
Gianluca Campanella |
| E-mail(s) |
g.campanella11@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Epidemiology and Biostatistics
|
| Street address |
Norfolk Place
|
| City |
London |
| ZIP/Postal code |
W2 1PG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE51032 |
EPIC-Italy at HuGeF |
| GSE51057 |
Methylome Analysis and Epigenetic Changes Associated with Menarcheal Age |
|
