|
| Status |
Public on Dec 31, 2013 |
| Title |
8412 D Aorta SMC 21 days |
| Sample type |
RNA |
| |
|
| Source name |
Aorta SMC 21 days
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: smooth muscle cell
developmental stage: embryo 21 days
tissue: Aorta
strain: wistar
|
| Treatment protocol |
Isolation of the fetal thorax was carefully performed in RNase free conditions, frozen in tissue tek, and stored at -80 C.
|
| Growth protocol |
Pups from 6 timed–pregnant Wistar rats were delivered after respectively 18 and 21 days of gestation (spontaneous birth occurs at 22 days of gestation) by hysterotomy through a median abdominal incision
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from microdissected cells was isolated using the RNeasy Minelute protocol.
|
| Label |
biotin
|
| Label protocol |
Two rounds of linear mRNA amplification were performed (modified Eberwine protocol). The second round of amplification, starting from 100 ng of first round aRNA, was finalised during the IVT labelling.
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to GeneChip Rat Genome 230 2.0 Array. Hybridization was performed using 12.5 μg of biotin-labeled RNA at 45°C for 16 hours under continuous rotation.
|
| Scan protocol |
Gene Chips were scanned using the Affymetrix Gene Chip Scanner 3000
|
| Data processing |
The Affymetrix probe level data were summarized using FARMS (Factor Analysis for Robust Microarray Summarization - http://www.bioinf.jku.at/software/farms/farms.html). Raw intensities were log2 transformed to get data normally distributed.
|
| |
|
| Submission date |
Sep 27, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Regina Bokenkamp |
| E-mail(s) |
R.Bokenkamp@lumc.nl
|
| Organization name |
LUMC
|
| Department |
Dept of Pediatric Cardiology
|
| Lab |
Anatomy & Embryology
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL17780 |
| Series (1) |
| GSE51248 |
Dlx1 and Rgs5 in the Ductus Arteriosus: Vessel-specific Genes Identified by Transcriptional Profiling of Laser-capture Microdissected Endothelial and Smooth Muscle Cells |
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