|
| Status |
Public on Oct 28, 2013 |
| Title |
p25_12h exp2 |
| Sample type |
RNA |
| |
|
| Source name |
OLN cells transfected with p25 for 12 hrs
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: oligodendroglial cell line stably expressing alpha-synuclein
genotype/variation: expressing alpha-synuclein and p25alpha
time: 12 hrs
|
| Treatment protocol |
Cells stably expressing alpha-synuclein were transfected (using Fugene-6 transfection reagent) with an empty control vector or p25alpha pcDNA3.1 for 8, 12, or 16 hrs
|
| Growth protocol |
Cells were grown at 37C for 24 hrs prior to transfection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of total RNA was perfomed using Rneasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Biotinylated cRNAs (15 µg) generated from independent cell culture preparations were hybridized onto individual Affymetrix RAE230 2.0 oligonucleotide arrays for 16 h at 45°C (Affymetrix) according to the manufacturer´s protocol
|
| Scan protocol |
The arrays were scanned with a Hewlett-Packard Gene Array 3000 7G Scanner
|
| Description |
Gene expression data from OLN cells expressing alpha-synuclein and p25alpha - RNA isolated 12 hrs after transfection
|
| Data processing |
The data were analyzed using Microarray Suite version 5.0 (MAS 5.0) (Affymetrix)
|
| |
|
| Submission date |
Oct 28, 2013 |
| Last update date |
Oct 28, 2013 |
| Contact name |
Christine Lund Kragh |
| E-mail(s) |
clkragh@hotmail.com
|
| Organization name |
Aarhus University
|
| Department |
Biomedicine
|
| Street address |
Ole Worms Alle 1.170
|
| City |
Aarhus C |
| ZIP/Postal code |
8000 C |
| Country |
Denmark |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE51781 |
Expression data from oligodendroglial cell line |
|
