|
Status |
Public on Aug 01, 2014 |
Title |
HCT116 3BKO 5-Aza-CdR D5 |
Sample type |
genomic |
|
|
Source name |
Colon
|
Organism |
Homo sapiens |
Characteristics |
gender: Male cell line: HCT116 genotype: DNMT3B KO treatment: 5-Aza-CdR time: D5
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified by phenol chloroform extractions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation HM450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
colorectal carcinoma cell line;HCT116 derivative cell lines with a genetic knockout of DNMT3B SAMPLE 16
|
Data processing |
BeadStudio software v3.2
|
|
|
Submission date |
Oct 28, 2013 |
Last update date |
Aug 01, 2014 |
Contact name |
Xiaojing Yang |
E-mail(s) |
xiaojiny@usc.edu
|
Phone |
(323)865-0740
|
Organization name |
USC/Norris Comprehensive Cancer Center
|
Street address |
1441 Eastlake Ave, Room 7336
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90033 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE51810 |
Gene Body Methylation Directly Increases Gene Expression and is a Therapeutic Target for Genes Upregulated in Cancer (methylation) |
GSE51815 |
Gene Body Methylation Directly Increases Gene Expression and is a Therapeutic Target for Genes Upregulated in Cancer |
|