|
Status |
Public on Jan 15, 2014 |
Title |
102-CONT-2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
embryos_72 hpf_control morpholino
|
Organism |
Danio rerio |
Characteristics |
strain: TL genotype/variation: wild type developmental stage: 72 hpf embryo injected with: control morpholino oligonucleotides
|
Treatment protocol |
Morpholino oligonucleotides(Control, AHRRa or AHRRb) were injected into 1-2 cell stage zebrafish embryos and sampled at 72 hpf for microarray analysis
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using STAT60 protocol
|
Label |
Cy5
|
Label protocol |
Labeling was done using Agilent Low Input Quick Amp Labeling kit
|
|
|
Channel 2 |
Source name |
universal reference RNA
|
Organism |
Danio rerio |
Characteristics |
strain: TL genotype/variation: wild type sample type: universal reference RNA
|
Treatment protocol |
Morpholino oligonucleotides(Control, AHRRa or AHRRb) were injected into 1-2 cell stage zebrafish embryos and sampled at 72 hpf for microarray analysis
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using STAT60 protocol
|
Label |
Cy3
|
Label protocol |
Labeling was done using Agilent Low Input Quick Amp Labeling kit
|
|
|
|
Hybridization protocol |
Equal amount of Cy3 (Universal Reference RNA) and Cy5 (individual samples) labeled cRNA probes were mixed and hybridized
|
Scan protocol |
Genepix 4100A scanner
|
Description |
Sample 2
|
Data processing |
Feature Extraction software and data processing using Limma, bioconductor package
|
|
|
Submission date |
Nov 08, 2013 |
Last update date |
Jan 15, 2014 |
Contact name |
Neelakanteswar Aluru |
E-mail(s) |
naluru@whoi.edu
|
Phone |
508-289-3607
|
Organization name |
Woods Hole Oceanographic Institution
|
Department |
Biology
|
Lab |
Aluru Lab
|
Street address |
45 Water Street
|
City |
Woods Hole |
State/province |
MA |
ZIP/Postal code |
02543 |
Country |
USA |
|
|
Platform ID |
GPL7302 |
Series (1) |
GSE52229 |
Knockdown of a zebrafish aryl hydrocarbon receptor repressor (ahrra) affects expression of genes related to photoreceptor development and hematopoiesis. |
|