|
| Status |
Public on Nov 13, 2013 |
| Title |
StromaLTbR-KO_fresh_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: splenic stroma
strain: C57BL/6
genotype: LTbR-/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol. RNA was quantified using Nanodrop and its quality was assessed by Agilent Total RNA 6000 chip.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with Illumina® TotalPrep™ RNA Amplification Kit (Ambion®)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
G
|
| Data processing |
After background substitution the data were normalized using cubic spline normalization with Genome Studio
|
| |
|
| Submission date |
Nov 12, 2013 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Marina Alekseevna Afanasyeva |
| E-mail(s) |
m.a.afanasyeva@gmail.com
|
| Phone |
+79263077071
|
| Organization name |
Engelhardt Institute of Molecular Biology, RAS
|
| Lab |
laboratory of immunoregulation
|
| Street address |
Vavilova 32
|
| City |
Moscow |
| ZIP/Postal code |
119991 |
| Country |
Russia |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE52294 |
Genome-wide analysis of gene expression in mouse spleen stroma |
|
