|
Status |
Public on Nov 16, 2013 |
Title |
SS-07 |
Sample type |
RNA |
|
|
Source name |
tumor sample
|
Organism |
Homo sapiens |
Characteristics |
gender: male age (yrs): 19 sarcoma subtype: synovial sarcoma (SS)
|
Treatment protocol |
All sarcoma samples of this set were obtained prior to any cytotoxic treatment and were snap-frozen immediately after surgery. Prior to RNA extraction tumor cell content was checked by a pathologist and only samples with >80% tumor content were processed further.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from snap-frozen tissue using Allprep DNA/RNA Mini Kit (Qiagen) according to manufacturer’s instructions. Quality control and quantification of total RNA was done with a RNA 6000 nano LabChip using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). Only RNA with a RNA integrity number > 7 was used.
|
Label |
biotin
|
Label protocol |
Expression profiling was performed using the HumanHT-12 v3 BeadArrays (Illumina) according to the manufacturers’ instructions. Quality control as well as labeling and hybridization were performed in the Core Facility of the DKFZ Heidelberg.
|
|
|
Hybridization protocol |
Expression profiling was performed using the HumanHT-12 v3 BeadArrays (Illumina) according to the manufacturers’ instructions.
|
Scan protocol |
Expression profiling was performed using the HumanHT-12 v3 BeadArrays (Illumina) according to the manufacturers’ instructions.
|
Data processing |
Annotation and quantile normalization were performed using a lumi R-package (Du P et al. BMC Bioinformatics 2010, 11:587.; Du P et al. Cancer 2007, 2:1-31.)
|
|
|
Submission date |
Nov 14, 2013 |
Last update date |
Nov 16, 2013 |
Contact name |
Marcus Renner |
E-mail(s) |
marcus.renner@med.uni-heidelberg.de
|
Organization name |
Institute of Pathology
|
Department |
General Pathology
|
Street address |
Im Neuenheimer Feld 224
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL6947 |
Series (2) |
GSE52390 |
Integrative DNA methylation and gene expression analysis in high-grade soft tissue sarcomas [gene expression] |
GSE52392 |
Integrative DNA methylation and gene expression analysis in high-grade soft tissue sarcomas |
|