|
| Status |
Public on Nov 01, 2021 |
| Title |
mutant, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow, mutant
|
| Organism |
Mus musculus |
| Characteristics |
background: B6D2F1
tissue: Bone marrow
condition: transgenic Plgf
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol (Life Technologies)
|
| Label |
biotin
|
| Label protocol |
cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
|
| |
|
| Hybridization protocol |
Hybridization was performed according to standard Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using the GeneChip 3000 7G Plus Affymetrix scanner
|
| Description |
Gene expression data from bone marrow cells, transgenic Plgf
|
| Data processing |
The data were normalized and background corrected using the RMA function in R/Bioconductor
|
| |
|
| Submission date |
Nov 15, 2013 |
| Last update date |
Nov 01, 2021 |
| Contact name |
Paolo Uva |
| Organization name |
IRCCS Istituto Giannina Gaslini
|
| Lab |
Clinical Bioinformatics
|
| Street address |
Via Gerolamo Gaslini, 5
|
| City |
Genoa |
| ZIP/Postal code |
16147 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE52414 |
Gene expression changes induced in mouse bone marrow by placenta growth factor (PlGF) overexpression. |
|
