|
| Status |
Public on Jun 20, 2014 |
| Title |
Imatinib 48h/Control 48h biological replica 3 technical replica 2 (dye swap) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Imatinib 48h 3_Cy3
|
| Organism |
Schistosoma mansoni |
| Characteristics |
developmental stage: adult
agent: Imatinib-treated
time: 48h
|
| Treatment protocol |
adult paired worms were treated with 50 µM Imatinib during 24h and 48h with the respectives controls (non-treated worms)
|
| Growth protocol |
worms were obtained by portal perfusion in 42-days infected hamsters
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from all samples was extract using Trizol reagent (invitrogen) followed by DNAse treatment using RNeasy kit (QIAGEN) according to manufacturer´s instructions.
|
| Label |
Cy3
|
| Label protocol |
All samples were linearly amplified and labeled using Agilent protocol with Low input RNA amplification kit
|
| |
|
| Channel 2 |
| Source name |
Control 48h 3_ Cy5
|
| Organism |
Schistosoma mansoni |
| Characteristics |
developmental stage: adult
agent: Non-treated
time: 48h
|
| Treatment protocol |
adult paired worms were treated with 50 µM Imatinib during 24h and 48h with the respectives controls (non-treated worms)
|
| Growth protocol |
worms were obtained by portal perfusion in 42-days infected hamsters
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from all samples was extract using Trizol reagent (invitrogen) followed by DNAse treatment using RNeasy kit (QIAGEN) according to manufacturer´s instructions.
|
| Label |
Cy5
|
| Label protocol |
All samples were linearly amplified and labeled using Agilent protocol with Low input RNA amplification kit
|
| |
|
| |
| Hybridization protocol |
Samples were combined and hybridized on a custom oligoarray with 4 x 44k elements produced by Agilent Technologies (GPL8606), the hybridization and washing steps were perfomed according to agilent instructions
|
| Scan protocol |
The washed slides were scanned using the equipament GenePix4000B (molecular devices)
|
| Description |
Imatinib 48h/Control 48h 3_s
ch1: Imatinib-treated paired adult worms (48h) biological replica 3- technical replica 2 _Cy3 (dye swap)
ch2: Non-treated paired adult worms (48h) biological replica 3 - technical replica 2_Cy5 (dye swap)
dye swap
|
| Data processing |
Data was extracted using Feature Extraction software (Agilent Technologies), the intensities were LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Dec 10, 2013 |
| Last update date |
Jun 21, 2014 |
| Contact name |
Katia Cristina Pereira Oliveira |
| Organization name |
Instituto Adolfo Lutz
|
| Department |
Centro de Parasitologia e Micologia
|
| Lab |
Nucleo de Enterparasitas
|
| Street address |
Av Prof lineu prestes 748
|
| City |
São Paulo |
| State/province |
O |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL8606 |
| Series (1) |
| GSE53154 |
Imatinib treatment causes substantial transcriptional changes in adult Schistosoma mansoni in vitro exhibiting pleiotropic effects |
|
