|
| Status |
Public on Mar 15, 2016 |
| Title |
Female_Placenta_Ko_1 |
| Sample type |
RNA |
| |
|
| Source name |
female mouse placenta (E16.5)
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: BAHD1 knockout
|
| Treatment protocol |
Total ablation of the BAHD1 gene.
|
| Growth protocol |
Placenta and embryonic livers of pregnant mice at embryonic day E16.5 were removed immediately after sacrifice, flash frozen in liquid nitrogen and stored at −80°C until further analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol reagent from biological replicates according to the manufacturer's instructions. RNA was treated with DNase I to remove residual DNA and then cleaned with DNase Inactivation Reagent (Ambion® Inc.) RNA was quality checked an then store at -80°C.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified with Ovation Pico WTA System v2 (Nugen Technologies, Inc. #3302-12), fragmented and biotin labeled using the Encore Biotin Module, (Nugen Technologies, Inc. #4200-12), according to the manufacturer's
|
| |
|
| Hybridization protocol |
Hybridization cocktail and post-hybridization processing was performed according to the “Target Preparation for Affymetrix GeneChip® Eukaryotic Array Analysis” protocol found in the appendix of the Nugen protocol of the fragmentation kit.
|
| Scan protocol |
scanned with an Affymetrix Genechip Scanner 3000, generating CEL files for each array.
|
| Description |
Gene expression data
|
| Data processing |
Data were normalized with RMA, analysed with LPE with three FDR methods; the z real value is indicated in the matrix.
|
| |
|
| Submission date |
Dec 18, 2013 |
| Last update date |
Mar 17, 2016 |
| Contact name |
Helene Bierne |
| E-mail(s) |
helene.bierne@inrae.fr
|
| Phone |
33134652289
|
| Organization name |
INRAE - University PARIS SACLAY
|
| Department |
Micalis Institute
|
| Lab |
Epigenetics and Cellular Microbiology
|
| Street address |
Domaine de Vilvert, bat 442, INRA Micalis
|
| City |
Jouy-en-Josas |
| ZIP/Postal code |
78350 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE53442 |
Transcriptome expression profile of embryonic liver and placenta tissue from BAHD1+/+ (WT) and BAHD1-/- (KO) mice at embryonic day E16.5 |
| GSE53443 |
Transcriptome expression profile of tissue from BAHD1+/+ (WT) and BAHD1-/- (KO) mice |
|
