|
| Status |
Public on Jan 14, 2014 |
| Title |
MEF 5F TGFb Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
MEFs treated with 5F in the presence of TGFb
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic fibroblast
treatment: 5F in the presence of TGFb
|
| Treatment protocol |
MEFs were plated into poly-L-lysine-coated 6 well dishes at 75,000 cells/well on Day -2, with 500 μL (≥ 2.5x105 IFU) each of rtTA lentivirus (FUdeltaGW-rtTA, Addgene plasmid 19780) and TroponinT-GCaMP-Zeo reporter lentivirus per well with 2 mL MEF medium. The following day (Day -1), culture medium was replaced with 2 mL fresh MEF medium with the molecules or DMSO vehicle and cells were transduced with 500 μL (≥ 2.5x105 IFU) of each tetO-transcription factor lentivirus. The next day (Day 0), medium was switched to 3 mL/well Reprogramming Medium consisting of AGM (Lonza CC-3186) without EGF, supplemented with 2 μg/mL doxycycline (Sigma).
|
| Growth protocol |
MEFs were grown in DMEM with 10% fetal bovine serum and 1X Glutamax.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected in Trizol at Day 3 post-transduction. Non-transduced MEFs were cultured and collected at Day 3. Samples were collected in triplicate from independent biological replicates and total RNA was prepared by the University of Pennsylvania Molecular Profiling Facility.
|
| Label |
biotin
|
| Label protocol |
The labeling, hybridization, and scanning procedures were performed by the University of Pennsylvania Molecular Profiling facility
|
| |
|
| Hybridization protocol |
The labeling, hybridization, and scanning procedures were performed by the University of Pennsylvania Molecular Profiling facility
|
| Scan protocol |
The labeling, hybridization, and scanning procedures were performed by the University of Pennsylvania Molecular Profiling facility
|
| Description |
MEF 5TF treatment TGFb
Gene expression data from TGFb treated MEFs that received 5TF treatment
|
| Data processing |
Initial data analysis was performed using Affymetrix Microarray Suite 5.0 and further analyzed using PartekGS software.
|
| |
|
| Submission date |
Jan 13, 2014 |
| Last update date |
Jan 14, 2014 |
| Contact name |
John Gearhart |
| E-mail(s) |
gearhart@upenn.edu
|
| Phone |
215-898-4450
|
| Organization name |
University of Pennsylvania
|
| Department |
Dept of Cell and Developmental Biology, Institute for Regenerative Medicine
|
| Street address |
3400 Civic Center Blvd, Building 421, 9-121 Smilow Center for Translational Research
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE54022 |
Inhibition of TGFβ Signaling Increases Direct Conversion of Fibroblasts to Induced Cardiomyocytes |
|
