|
| Status |
Public on Jan 24, 2014 |
| Title |
WAT CD14+ from Obese [49] |
| Sample type |
RNA |
| |
|
| Source name |
WAT CD14+ cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: WAT CD14+ cells
group: Diabetic
|
| Treatment protocol |
Adipocytes and cells of the stroma vascular fraction (SVF) were obtained by collagenase digestion of adipose tissue . SVF cells were resuspended in endotoxin-free PBS supplemented with 2% FCS and 1 mM EDTA. Isolation of CD14+ using positive selection magnetic beads (Stemcell technologies).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen Rneasy miKIT with DNASE treatment
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ovation picoSL V2 and Biotin IL from NuGen Inc for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Visceral Adipose tissue from patient unergoing gastric bypass surgery
Human subject
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI using Genome Studio (Illumina software)
|
| |
|
| Submission date |
Jan 23, 2014 |
| Last update date |
Jan 24, 2014 |
| Contact name |
Michele Guerre-Millo |
| E-mail(s) |
michele.guerre-millo@crc.jussieu.fr
|
| Phone |
33 1 44278072
|
| Organization name |
INSERM
|
| Department |
UMRS1166
|
| Lab |
Nutriomic
|
| Street address |
91 Boulevard de l'Hopital
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE54350 |
Genome wide analysis of Visceral adipose tissue CD14+ cells from Obese and obese diabetic subjects |
|
