|
| Status |
Public on Jan 24, 2014 |
| Title |
Cdk9_MM1S_DMSO_forCdk9i_ChipSeq |
| Sample type |
SRA |
| |
|
| Source name |
Multiple myeloma
|
| Organism |
Homo sapiens |
| Characteristics |
chip antibody: Cdk9
antibody catalog number: Santa Cruz SC-484
cell line: MM1.S
duration: 6h
drug: DMSO
|
| Treatment protocol |
Treated with DMSO (for 6 hours), use with Cdk9 inhibitor studies
|
| Growth protocol |
RPMI 1640 supplemented with 10% FCS and 1% GlutaMAX
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Chromatin IP against Cdk9_MM1S_DMSO_forCdk9i
barcode (already removed): GTGAAA
|
| Data processing |
Images analysis and base calling was done using the solexa pipeline.
For all samples reads were aligned to their indicated build using bowtie with parameters -e 70 -k 1 -m 1 -n 2 --best --concise. Seed length (-l) was set to read length for each dataset.
Genome_build: hg18
|
| |
|
| Submission date |
Jan 23, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE50622 |
Targeting transcriptional dependencies in cancer using a covalent CDK7 inhibitor (ChIP-Seq) |
| GSE50625 |
Targeting transcriptional dependencies in cancer using a covalent CDK7 inhibitor |
|
| Relations |
| BioSample |
SAMN02595584 |
| SRA |
SRX447603 |
