|
Status |
Public on Jun 01, 2014 |
Title |
Blood from Stage I (GP2477_WB_143055HP133A11) |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood age: 58 gender: M gold stage: Stage I plateid: 1 subject type: Smoker Controls hyb date: 39323
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
|
|
|
Hybridization protocol |
Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
Data processing |
The data was analysed with Rosetta Resolver version 5.0
|
|
|
Submission date |
Feb 10, 2014 |
Last update date |
Jun 01, 2014 |
Contact name |
steve matthew fox |
E-mail(s) |
steven.m.fox@gsk.com
|
Phone |
01438 763226
|
Organization name |
GlaxoSmithKline
|
Department |
Statistics
|
Street address |
Gunnels Wood Road
|
City |
Stevenage |
ZIP/Postal code |
SG1 2NY |
Country |
United Kingdom |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE54837 |
Altered gene expression in blood and sputum from COPD patients with frequent exacerbations |
|