|
| Status |
Public on Feb 28, 2014 |
| Title |
MD_MB_159 |
| Sample type |
genomic |
| |
|
| Source name |
Primary medulloblastoma tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
age (yrs): 11.0
tissue: Primary medulloblastoma tumor tissue
molecular subgroup: Group 3
sample type: formalin-fixed paraffin-embedded (FFPE)
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of high molecular weight DNA from frozen tumor samples was carried out using a Qiagen Allprep DNA/RNA/Protein Mini Kit.
|
| Label |
biotin/DNP
|
| Label protocol |
According to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
According to the manufacturer’s instructions
|
| Scan protocol |
Illumina iScan System, according to the manufacturer’s instructions
|
| Description |
MD_MB_159.AVG_Beta
|
| Data processing |
Array processing was performed using Illumina GenomeStudio V2011.1 (Methylation Module version 1.9.0). The 'MB-450k_GEOupload_Raw.txt' contains unmethylated and methylated signal intensities. Signal intensities were obtained without background subtraction and normalized to internal controls. The 'MB-450k_GEOupload_Beta.txt' contains 'Beta-values'.
|
| |
|
| Submission date |
Feb 11, 2014 |
| Last update date |
Feb 28, 2014 |
| Contact name |
Peter Lichter |
| Organization name |
German Cancer Research Center
|
| Department |
Molecular Genetics
|
| Street address |
Im Neuenheimer Feld 580
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE54880 |
Microarray-based DNA methylation profiling of medulloblastoma and normal cerebellum samples |
|
