|
| Status |
Public on Oct 01, 2006 |
| Title |
skin_normal_Healthy5 (U133A) |
| Sample type |
RNA |
| |
|
| Source name |
skin(dermis and epidermis)_upper leg
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue:skin(dermis and epidermis)_Source:upper leg_Health status:normal_Age:32years_Gender:male_Race:white
|
| Treatment protocol |
see Materials and Methods section of DA Plager, AA Leontovich, et al. Experimental Dermatology 2006 article; all therapeutic treatments or confounding exposures were avoided
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
see Materials and Methods section of DA Plager, AA Leontovich, et al. Experimental Dermatology 2006 article; Trizol extraction of total RNA followed by RNeasy clean-up of total RNA was performed according to the manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized overnight at 45C on Affymetrix Human Genome U133A GeneChip. GeneChips were washed and stained with phycoerythrin-labelled streptavidin in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner.
|
| Description |
no additional information
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) to quantify and scale the fluorescence data.
|
| |
|
| Submission date |
Aug 29, 2006 |
| Last update date |
Aug 29, 2006 |
| Contact name |
Douglas A Plager |
| E-mail(s) |
daplager@hotmail.com
|
| Organization name |
Mayo Clinic Rochester
|
| Department |
Dermatology
|
| Street address |
200 First Street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE5667 |
Transcription data from Normal Skin and Nonlesional and Lesional Atopic Dermatitis/Eczema Skin |
|
