|
| Status |
Public on Feb 14, 2014 |
| Title |
Thymus_Tcf-/-_mouse2 |
| Sample type |
RNA |
| |
|
| Source name |
Thymus, homogenized
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6 Tcf1−/− ΔVII/ΔVII
tissue: thymus
|
| Growth protocol |
direct isolation from homogenized thymus tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy minicolumns, accoding to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
One µg of RNA was used to generate cRNA using Affymetrix One cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA, USA), after which the samples were biotinylated using an Affymetrix IVT labeling kit.
|
| |
|
| Hybridization protocol |
The samples were hybridized overnight at 42°C to GeneChip mouse genome 430 2.0 Arrays (Affymetrix). Washing and staining steps were performed on a Fluidics station 450 (Affymetrix).
|
| Scan protocol |
Genechips were scanned using a GeneChip scanner 3000 (Affymetrix) according to the manufacturer's instructions.
|
| Data processing |
Raw data were normalized and summarized using Robust Multichip Average (RMA) method. Array analysis was performed using R-2.14 (http://cran.r-project.org/) and Bioconductor 2.9 software (http://www.bioconductor.org/)
|
| |
|
| Submission date |
Feb 13, 2014 |
| Last update date |
Feb 14, 2014 |
| Contact name |
Martijn H Brugman |
| E-mail(s) |
m.h.brugman@lumc.nl
|
| Phone |
+31-71-5261300
|
| Organization name |
LUMC
|
| Department |
IHB
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE54976 |
Gene expression in thymi of Tcf1 -/-, Tcf +/- or Tcf1 -/- mice with tumor. |
|
