|
| Status |
Public on Feb 19, 2014 |
| Title |
WT, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Ventral midbrain from WT mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Ventral midbrain
genotype: Wild-type
age: 10-15 weeks
genetic background: C57BL/6J
|
| Treatment protocol |
After entrainment for >10 days under LD conditions, mice were kept in constant darkness (DD) for 2 days starting at lights-off time. On the third day, mice were sacrificed at indicated time points by cervical dislocation. Tissue was rapidly removed and frozen in liquid nitrogen.
|
| Growth protocol |
Male RKO and WT mice (10-15 weeks of age) were maintained in a C57BL/6J background. Mice were housed in temperature-controlled (22-23ºC) quarters under a 12-h light-dark (LD) photoperiod (lights on at 8:00 a.m.).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by the single-step acid guanidinium thiocyanate-phenol-chloroform method.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from ventral midbrain of WT mice
|
| Data processing |
The data were analyzed with GCOS software using Affymetrix default analysis settings and normalized by RMA (Robust Multi-array Average) method.
|
| |
|
| Submission date |
Feb 18, 2014 |
| Last update date |
Feb 19, 2014 |
| Contact name |
Gi Hoon Son |
| E-mail(s) |
songh@korea.ac.kr
|
| Phone |
8229206147
|
| Organization name |
Korea University
|
| Department |
Department of Legal Medicine
|
| Street address |
Inchon-ro 73
|
| City |
Seoul |
| State/province |
select a state |
| ZIP/Postal code |
136-705 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE55119 |
Expression data from mouse ventral midbrain |
|
