|
| Status |
Public on Nov 14, 2014 |
| Title |
MHO7 |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous adipose fasting
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Subcutaneous adipose
disease state: metabolically healthy obese MHO
|
| Treatment protocol |
Samples were collected after an overnight fast (12h)
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted after homogenisation in TRIzol with a tissue homogenizer and using the Qiagen RNeasy Mini Kit (Qiagen, Mississauga, ON, Canada), according to manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 100 ng total RNA according to the standard Affymetrix protocol (WT Expression Kit User Manual, 2010, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA was hybridized for 20 hr on Human Gene 2.1 ST arrays incubated at 48C. GeneChips were washed and stained using the Gene Atlas Fluidics Station.
|
| Scan protocol |
HuGene 2.1 ST arrays were scanned using the Gene Atlas Imaging Station.
|
| Description |
Gene expression data from human periumbilical subcutaneous tissue
DRA_21
|
| Data processing |
Data was analyzed with R/Bioconductor software and normalized using RMA. An ANOVA was used to identify differentially expressed genes. An FDR of 0.05 was used to account for multiple testing.
|
| |
|
| Submission date |
Feb 20, 2014 |
| Last update date |
Nov 15, 2014 |
| Contact name |
David M Mutch |
| E-mail(s) |
dmutch@uoguelph.ca
|
| Phone |
+1-519-824-4120
|
| Organization name |
University of Guelph
|
| Department |
Human Health & Nutritional Sciences
|
| Lab |
ANNU 308
|
| Street address |
50 Stone Road East
|
| City |
Guelph |
| State/province |
Ontario |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE55200 |
Gene expression data from human subcutaneous adipose tissue |
|
