|
Status |
Public on Jan 22, 2015 |
Title |
Peripheral blood, 7471147065_R05C01 |
Sample type |
genomic |
|
|
Source name |
peripheral blood
|
Organism |
Homo sapiens |
Characteristics |
tissue: peripheral blood dataset: population study gender: M age: 35.3
|
Treatment protocol |
N/A
|
Growth protocol |
N/A
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Bisulfite conversion of genomic DNA from peripheral blood was performed using the EZ DNA methylation kit according to manufacturer's instructions (Zymo Research).
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol.
|
|
|
Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina HumanMethylation450 BeadChip using standard Illumina protocol.
|
Scan protocol |
Illumina HiScan using standard recommended Illumina scanner setting.
|
Description |
Population study sample.
|
Data processing |
Custom R scripts. Quantile normalisation of signal intensities.
|
|
|
Submission date |
Mar 10, 2014 |
Last update date |
Mar 03, 2015 |
Contact name |
Benjamin Lehne |
E-mail(s) |
b.lehne@imperial.ac.uk
|
Organization name |
Imperial College London
|
Department |
Epidemiology and Biostatistics
|
Street address |
Medicine, Norfolk Place, St Mary's Campus
|
City |
London |
ZIP/Postal code |
W2 1PG |
Country |
United Kingdom |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE55763 |
A coherent approach for analysis of the Illumina HumanMethylation450 BeadChip improves data quality and performance in epigenome-wide association studies |
|