|
| Status |
Public on Jan 22, 2015 |
| Title |
Peripheral blood, 7471147067_R04C01 |
| Sample type |
genomic |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
dataset: population study
gender: M
age: 52.5
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Bisulfite conversion of genomic DNA from peripheral blood was performed using the EZ DNA methylation kit according to manufacturer's instructions (Zymo Research).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol.
|
| |
|
| Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina HumanMethylation450 BeadChip using standard Illumina protocol.
|
| Scan protocol |
Illumina HiScan using standard recommended Illumina scanner setting.
|
| Description |
Population study sample.
|
| Data processing |
Custom R scripts. Quantile normalisation of signal intensities.
|
| |
|
| Submission date |
Mar 10, 2014 |
| Last update date |
Mar 03, 2015 |
| Contact name |
Benjamin Lehne |
| E-mail(s) |
b.lehne@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Epidemiology and Biostatistics
|
| Street address |
Medicine, Norfolk Place, St Mary's Campus
|
| City |
London |
| ZIP/Postal code |
W2 1PG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE55763 |
A coherent approach for analysis of the Illumina HumanMethylation450 BeadChip improves data quality and performance in epigenome-wide association studies |
|
