|
| Status |
Public on Dec 01, 2014 |
| Title |
KO mice Islet sample No.3 |
| Sample type |
RNA |
| |
|
| Source name |
RasGrf1 Islets 3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pancreatic islets
genotype: RasGrf1 KO
genetic background: C57BL/6N
|
| Growth protocol |
Animals of the same sex were housed in type IIL individually ventilated cages, in a temperature- and humidity-controlled room with a 12h light/dark cycle, with food (Teklad 2014, Harlan Laboratories) and water available ad libitum. All testing was completed during the light phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Manually selected pancreatic islets from collagenase-P digests of WT and RasGrf1 KO mice pancreases were used for RNA extraction using the TRIZOL protocol. Trizol extraction of total RNA was performed according to the manufacturer's instructions. Further RNA purification was performed usin Rneasy mini kit from Qiagen.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
The RMA function provided by the package Affy from R/Bioconductor was applied to the raw expression data.
|
| |
|
| Submission date |
Mar 31, 2014 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Alberto Fernández-Medarde |
| E-mail(s) |
afm@usal.es
|
| Organization name |
Instituto de Biología Molecular y Celular del Cáncer-IBMCC
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| State/province |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE56371 |
Transcriptional profiling reveals functional links between RasGrf1 and Pttg1 in pancreatic beta cells |
|
