|
| Status |
Public on Jan 23, 2015 |
| Title |
DNA from PBMC P14, baseline |
| Sample type |
genomic |
| |
|
| Source name |
PBMCs, before rhGH treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral blood mononuclear cells
treatment: none
patient: 14
|
| Treatment protocol |
4 days of rhGH treatment during IGF-1 generation test.
|
| Growth protocol |
None.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNeasy Blood and Tissue Kit (Qiagen).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol.
|
| |
|
| Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol.
|
| Scan protocol |
Arrays were scanned using the Illumina iScan.
|
| Description |
P14_baseline
IGF-1 generation test
|
| Data processing |
GenomeStudio software (version 2011.1, Methylation module version 1.9.0).
|
| |
|
| Submission date |
Apr 25, 2014 |
| Last update date |
Jan 23, 2015 |
| Contact name |
Susanne Bens |
| E-mail(s) |
sbens@medgen.uni-kiel.de
|
| Organization name |
Christian-Albrechts-University Kiel & University Hospital Schleswig-Holstein
|
| Department |
Institute of Human Genetics
|
| Street address |
Arnold-Heller-Str.3 (Haus 10)
|
| City |
Kiel |
| ZIP/Postal code |
D-24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE57107 |
In vivo investigations of the effect of short- and long-term recombinant growth hormone treatment on DNA-methylation in humans |
| GSE57205 |
In vivo investigations of the effect of short- and long-term recombinant growth hormone treatment on DNA-methylation in humans |
|
