|
| Status |
Public on Jun 19, 2014 |
| Title |
H1975 cells, erlotinib 1 μM, 12 h |
| Sample type |
RNA |
| |
|
| Source name |
NCI-H1975 human lung non-small cell adenocarcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: erlotinib 1 μM, 12 h
cell line: NSCLC
genotype/variation: mutant EGFR (L858R/T790M)
|
| Treatment protocol |
Cells were treated with 1 μM erlotinib, 3 μM or 5 μM quinacrine or a combination of both for 0, 6, 12, 24, or 48 h.
|
| Growth protocol |
Cells were maintained in DMEM medium supplemented with 5% fetal bovine serum. All cells were kept at 37°C in a humidified atmosphere with 5% CO2. After 2 or 3 passages, cells were plated at 50-70% confluency in 10 cm tissue culture dishes for treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cells using the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit from Affymetrix at the Gene Expression & Genotyping Core Facility at Case Comprehensive Cancer Center.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix GeneTitan kit materials. Following fragmentation, samples were hybridized for 16 h at 45°C on Human Affymetrix Gene ST 2.1 array.
|
| Scan protocol |
Samples were scanned and digital images were archived using the Affymetrix GeneTitan MC automated system.
|
| Description |
erlotinib 1 μM, 12 h
|
| Data processing |
Raw CEL files were pre-processed using the Affymetrix Expression Console Software 1.30 with Robust Multi-array Average (RMA) normalization.
HuGene-2_1-st.pgf
HuGene-2_1-st.mps
|
| |
|
| Submission date |
May 07, 2014 |
| Last update date |
Mar 31, 2015 |
| Contact name |
George Stark |
| E-mail(s) |
starkg@ccf.org
|
| Organization name |
Cleveland Clinic Lerner Research Institute
|
| Department |
Cancer Biology
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE57422 |
Quinacrine Overcomes Resistance to Erlotinib by Inhibiting FACT, Nuclear Factor-kappa B and Cell Cycle Progression in Non-Small Cell Lung Cancer |
|
