|
| Status |
Public on May 27, 2014 |
| Title |
Hepatocytes_Microcystin-LR_.07 uM_1 d_Rep3_93468.CEL |
| Sample type |
RNA |
| |
|
| Source name |
Hepatocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Primary rat hepatocytes
compound: Microcystin-LR
dose: 0.07 uM
time: 1 d
vehicle: DMSO
rna extraction date: 38685
hybridization date: 38698
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Poly A(+) RNA was isolated using the MagNA Pure LC robot (Roche, Basel, Switzerland) in combination with the MagNA Pure LC mRNA Isolation kit I (Roche, Basel, Switzerland), concentrated by ethanol precipitation, resuspend in 7 ul DEPC-treated water, quantified by ribogreen fluorescence using Wallac Victor2 fluorometer (Perkin Elmer, Fremont CA) and transcribed to cDNA
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
Complete Drug Matrix dataset for primary rat hepatocytes; more details can be found at http://cebs.niehs.nih.gov/ under accession number 004-00008-0000-000-2
|
| Data processing |
Normalized expression of probe level data was calculated using the Robust Multiarray Average function (RMA)
|
| |
|
| Submission date |
May 19, 2014 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Scott Auerbach |
| E-mail(s) |
AuerbachS@niehs.nih.gov
|
| Organization name |
Division of the National Toxicology Program at NIEHS
|
| Street address |
111 TW Alexander Drive
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE57805 |
In vitro exposure of rat hepatocytes to a variety of toxicants, assayed by Affymetrix microarray |
| GSE57822 |
Drug Matrix Data - Affymetrix Arrays |
|
