|
| Status |
Public on Aug 01, 2014 |
| Title |
Keratinocyte proliferating vehicle 24 h rep2 |
| Sample type |
RNA |
| |
|
| Source name |
keratinocyte
|
| Organism |
Homo sapiens |
| Characteristics |
primary cells: Keratinocytes
treatment: vehicle 24h
|
| Treatment protocol |
Vehicle (ethanol), RA in ethanol or ddRA in ethanol was added to the cells (the final concentration of ethanol being 0.001%).
|
| Growth protocol |
Proliferating keratinocytes were grown in EpiLife medium containing HKGS+GA and used at 30-40% confluency. Cells induced to differentiate was at 80% confluency when switched to EpiLife medium (without rhEGF) supplemented with 1.5 mM CaCl2 for 4 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TriReagent (Ambion).
|
| Label |
Biotin
|
| Label protocol |
250 nanograms of total RNA from each sample were used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip® WT PLUS Reagent Kit User Manual ( P/N 703174 Rev 1 Affymetrix Inc., Santa Clara, CA)
|
| |
|
| Hybridization protocol |
GeneChip® Human Gene 2.1 ST Arrays were hybridized for 16 hours in a 45°C incubator, followed by washing and staining
|
| Scan protocol |
The GeneChip® Array-plates were scanned with GeneTitan® Multi-Channel (MC) Instrument
|
| Data processing |
The raw data was normalized in Expression Console provided by Affymetrix, using the robust multi-array average (RMA) method.
|
| |
|
| Submission date |
Jun 23, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Hans Törmä |
| E-mail(s) |
hans.torma@medsci.uu.se
|
| Organization name |
Uppsala University
|
| Department |
Dept of Medical Sciences
|
| Lab |
Rudbeck Laboratory/Dermatology
|
| Street address |
Dag Hammarskjölds väg 20
|
| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE58749 |
Human proliferating and differentiating keratinocytes treated with retinoic acid or 3,4-didehydroretinoic acid |
|
