|
Status |
Public on Aug 01, 2014 |
Title |
Keratinocyte proliferating ddRA 24 h rep1 |
Sample type |
RNA |
|
|
Source name |
keratinocyte
|
Organism |
Homo sapiens |
Characteristics |
primary cells: Keratinocytes treatment: ddRA 24h
|
Treatment protocol |
Vehicle (ethanol), RA in ethanol or ddRA in ethanol was added to the cells (the final concentration of ethanol being 0.001%).
|
Growth protocol |
Proliferating keratinocytes were grown in EpiLife medium containing HKGS+GA and used at 30-40% confluency. Cells induced to differentiate was at 80% confluency when switched to EpiLife medium (without rhEGF) supplemented with 1.5 mM CaCl2 for 4 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using TriReagent (Ambion).
|
Label |
Biotin
|
Label protocol |
250 nanograms of total RNA from each sample were used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip® WT PLUS Reagent Kit User Manual ( P/N 703174 Rev 1 Affymetrix Inc., Santa Clara, CA)
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|
|
Hybridization protocol |
GeneChip® Human Gene 2.1 ST Arrays were hybridized for 16 hours in a 45°C incubator, followed by washing and staining
|
Scan protocol |
The GeneChip® Array-plates were scanned with GeneTitan® Multi-Channel (MC) Instrument
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Data processing |
The raw data was normalized in Expression Console provided by Affymetrix, using the robust multi-array average (RMA) method.
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|
|
Submission date |
Jun 23, 2014 |
Last update date |
Aug 01, 2014 |
Contact name |
Hans Törmä |
E-mail(s) |
hans.torma@medsci.uu.se
|
Organization name |
Uppsala University
|
Department |
Dept of Medical Sciences
|
Lab |
Rudbeck Laboratory/Dermatology
|
Street address |
Dag Hammarskjölds väg 20
|
City |
Uppsala |
ZIP/Postal code |
75185 |
Country |
Sweden |
|
|
Platform ID |
GPL17692 |
Series (1) |
GSE58749 |
Human proliferating and differentiating keratinocytes treated with retinoic acid or 3,4-didehydroretinoic acid |
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