|
Status |
Public on Mar 02, 2015 |
Title |
G051: Prostate_Cancer |
Sample type |
RNA |
|
|
Source name |
radical prostatectomy prostate tumor
|
Organism |
Homo sapiens |
Characteristics |
gleason score: 3+3 pt-stage: 3c pre-treatment psa (µg/l): - psa progression: yes development of metastasis: no surgical margins: negative
|
Treatment protocol |
Samples were taken from the surgically removed prostate / lymph node and fresh frozen in liq N2-cooled iso-pentane
|
Growth protocol |
Samples were directly taken from the patient tissues
|
Extracted molecule |
total RNA |
Extraction protocol |
The standard protocol from RNA-Bee (Campro Scientific) was used to extract total RNA
|
Label |
biotin
|
Label protocol |
Standard whole transcript labeling protocol from Affymetrix human exon 1.0 ST
|
|
|
Hybridization protocol |
Standard hybridization protocol for Affymetrix human exon 1.0 ST
|
Scan protocol |
Standard scanner for Affymetrix human exon 1.0 ST
|
Data processing |
Cel files were processed using R-package aroma.affymetrix RMA full probeset level chip-definition_file: HuEx-1_0-st-v2,fullR3,A20071112,EP.cdf
|
|
|
Submission date |
Jul 24, 2014 |
Last update date |
Mar 02, 2015 |
Contact name |
Guido Jenster |
E-mail(s) |
g.jenster@erasmusmc.nl
|
Phone |
31107043672
|
Organization name |
Erasmus MC
|
Department |
Urology
|
Lab |
JNI, Be362a
|
Street address |
P.O. Box 2040
|
City |
Rotterdam |
ZIP/Postal code |
3000CA |
Country |
Netherlands |
|
|
Platform ID |
GPL5175 |
Series (1) |
GSE59745 |
Identification of novel long non-coding RNAs in prostate cancers |
|