|
| Status |
Public on Mar 02, 2015 |
| Title |
G326: Lymph_node_metastasis |
| Sample type |
RNA |
| |
|
| Source name |
lymph node metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
gleason score: 5+4
pt-stage: -
pre-treatment psa (µg/l): -
psa progression: no
development of metastasis: no
surgical margins: -
|
| Treatment protocol |
Samples were taken from the surgically removed prostate / lymph node and fresh frozen in liq N2-cooled iso-pentane
|
| Growth protocol |
Samples were directly taken from the patient tissues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The standard protocol from RNA-Bee (Campro Scientific) was used to extract total RNA
|
| Label |
biotin
|
| Label protocol |
Standard whole transcript labeling protocol from Affymetrix human exon 1.0 ST
|
| |
|
| Hybridization protocol |
Standard hybridization protocol for Affymetrix human exon 1.0 ST
|
| Scan protocol |
Standard scanner for Affymetrix human exon 1.0 ST
|
| Data processing |
Cel files were processed using R-package aroma.affymetrix RMA full probeset level
chip-definition_file: HuEx-1_0-st-v2,fullR3,A20071112,EP.cdf
|
| |
|
| Submission date |
Jul 24, 2014 |
| Last update date |
Mar 02, 2015 |
| Contact name |
Guido Jenster |
| E-mail(s) |
g.jenster@erasmusmc.nl
|
| Phone |
31107043672
|
| Organization name |
Erasmus MC
|
| Department |
Urology
|
| Lab |
JNI, Be362a
|
| Street address |
P.O. Box 2040
|
| City |
Rotterdam |
| ZIP/Postal code |
3000CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE59745 |
Identification of novel long non-coding RNAs in prostate cancers |
|
