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| Status |
Public on Jul 26, 2014 |
| Title |
parental P1152 total RNA |
| Sample type |
SRA |
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| Source name |
parental P1152
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| Organism |
Drosophila melanogaster |
| Characteristics |
age: 1-2 week old
strain: parental P1152
tissue: ovary
antibody: none
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| Extracted molecule |
total RNA |
| Extraction protocol |
ovaries dissected, for IP samples: immunoprecipitation of Argonaute protein, extraction of small RNA, cloning of library. For total RNA libraries: isolation of total RNA after ovary dissection and cloning of small RNA library
custom made protocol for cloning of piRNAs: isolation of small RNAs on acrylamide Gel or through IP of Argonaute, followed by 3' ligration, 5' ligation, Reverse transcription and final PCR amplification
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| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
total small RNA
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| Data processing |
mapping with Bowtie to dm3
Genome_build: dm3
Supplementary_files_format_and_content: txt file containing tab delimited sequence, chromosome, start, stop, annotation and frequency
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| Submission date |
Jul 25, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Adrien Le Thomas |
| Organization name |
California Institute of Technology
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| Department |
Biology and Biological Engineering
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| Lab |
Aravin
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| Street address |
231 South Hudson Avenue
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| City |
Pasadena |
| State/province |
CA - California |
| ZIP/Postal code |
91101 |
| Country |
USA |
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| Platform ID |
GPL13304 |
| Series (2) |
| GSE59606 |
Trans-generationally inherited piRNAs trigger piRNA biogenesis by changing the chromatin of piRNA clusters and inducing precursor processing [small RNA-IP] |
| GSE59610 |
Trans-generationally inherited piRNAs trigger piRNA biogenesis by changing the chromatin of piRNA clusters and inducing precursor processing |
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| Relations |
| BioSample |
SAMN02940629 |
| SRA |
SRX661244 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1446112_NR58_processed.txt.gz |
25.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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